Application | Comment | Organism |
---|---|---|
diagnostics | glucose sensor/glucose determination | Burkholderia cepacia |
Cloned (Comment) | Organism |
---|---|
expressed as recombinant protein in Escherichia coli strain DH5alpha | Burkholderia cepacia |
expression in Escherichia coli | Burkholderia cepacia |
Protein Variants | Comment | Organism |
---|---|---|
A472C | site directed mutagenesis | Burkholderia cepacia |
A472D | site directed mutagenesis | Burkholderia cepacia |
A472E | site directed mutagenesis | Burkholderia cepacia |
A472F | site directed mutagenesis | Burkholderia cepacia |
A472F | mutant with higher substrate specificity for glucose in relation to maltose, constructed by site-directed mutagenesis | Burkholderia cepacia |
A472G | site directed mutagenesis | Burkholderia cepacia |
A472H | site directed mutagenesis | Burkholderia cepacia |
A472I | site directed mutagenesis | Burkholderia cepacia |
A472K | site directed mutagenesis | Burkholderia cepacia |
A472L | site directed mutagenesis | Burkholderia cepacia |
A472M | site directed mutagenesis | Burkholderia cepacia |
A472N | site directed mutagenesis | Burkholderia cepacia |
A472P | site directed mutagenesis | Burkholderia cepacia |
A472Q | site directed mutagenesis | Burkholderia cepacia |
A472R | site directed mutagenesis | Burkholderia cepacia |
A472S | site directed mutagenesis | Burkholderia cepacia |
A472T | site directed mutagenesis | Burkholderia cepacia |
A472V | site directed mutagenesis | Burkholderia cepacia |
A472W | site directed mutagenesis | Burkholderia cepacia |
A472Y | site directed mutagenesis | Burkholderia cepacia |
N475A | site directed mutagenesis | Burkholderia cepacia |
N475C | site directed mutagenesis | Burkholderia cepacia |
N475D | site directed mutagenesis | Burkholderia cepacia |
N475D | mutant with higher substrate specificity for glucose in relation to maltose, constructed by site-directed mutagenesis | Burkholderia cepacia |
N475E | site directed mutagenesis | Burkholderia cepacia |
N475F | site directed mutagenesis | Burkholderia cepacia |
N475G | site directed mutagenesis | Burkholderia cepacia |
N475H | site directed mutagenesis | Burkholderia cepacia |
N475I | site directed mutagenesis | Burkholderia cepacia |
N475K | site directed mutagenesis | Burkholderia cepacia |
N475L | site directed mutagenesis | Burkholderia cepacia |
N475M | site directed mutagenesis | Burkholderia cepacia |
N475P | site directed mutagenesis | Burkholderia cepacia |
N475Q | site directed mutagenesis | Burkholderia cepacia |
N475R | site directed mutagenesis | Burkholderia cepacia |
N475S | site directed mutagenesis | Burkholderia cepacia |
N475T | site directed mutagenesis | Burkholderia cepacia |
N475V | site directed mutagenesis | Burkholderia cepacia |
N475W | site directed mutagenesis | Burkholderia cepacia |
N475Y | site directed mutagenesis | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
D-glucose | A472F mutant, higher specificity toward glucose compared to the wild type enzyme, no activity towards maltose | Burkholderia cepacia | |
17 | - |
glucose | FADGDH mutant A472F | Burkholderia cepacia | |
28 | - |
D-glucose | N475D mutant, higher specificity toward glucose compared to the wild type enzyme, no activity towards maltose | Burkholderia cepacia | |
28 | - |
glucose | FADGDH mutant N475D | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia cepacia |
by DEAE-5PW and Superdex-200 10/300GL column chromatography | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
590 micromol/min/mg Vmax for the FADGDH mutant A472F | Burkholderia cepacia |
additional information | - |
770 micromol/min/mg Vmax for the FADGDH mutant N475D | Burkholderia cepacia |
4 | - |
A472F mutant towards maltose | Burkholderia cepacia |
21 | - |
N475D mutant towards maltose | Burkholderia cepacia |
118 | - |
N475D mutant towards glucose | Burkholderia cepacia |
120 | - |
A472F mutant towards glucose | Burkholderia cepacia |
139 | - |
wild type towards maltose | Burkholderia cepacia |
430 | - |
enzymatic activity of wild-type FADGDH in an assay with 40 mM maltose | Burkholderia cepacia |
543 | - |
wild type towards glucose | Burkholderia cepacia |
950 | - |
enzymatic activity of wild-type FADGDH in an assay with 40 mM glucose | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindophenol | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + acceptor | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced acceptor | - |
? | |
D-maltose + 2,6-dichlorophenolindophenol | - |
Burkholderia cepacia | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-maltose + acceptor | - |
Burkholderia cepacia | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD-dependent glucose dehydrogenase | - |
Burkholderia cepacia |
FAD-dependent glucose dehydrogenase | FADGDH | Burkholderia cepacia |
FADGDH | - |
Burkholderia cepacia |
glucose dehydrogenase | - |
Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Burkholderia cepacia |